ID   SACAT      standard; DNA; PRO; 901 BP.
XX
AC   X65462; S50737;
XX
NI   g49071
XX
DT   04-DEC-1992 (Rel. 34, Created)
DT   19-MAR-1997 (Rel. 51, Last updated, Version 9)
XX
DE   S.agalactiae plasmid pIP501 cat gene for chloramphenicol
DE   acetyltransferase
XX
KW   chloramphenicol acetyltransferase.
XX
OS   Streptococcus agalactiae
OC   Eubacteria; Firmicutes; Low G+C gram-positive bacteria;
OC   Streptococcaceae; Streptococcus.
XX
OG   Plasmid
XX
RN   [1]
RX   MEDLINE; 93096867.
RA   Trieu-Cuot P., de Cespedes G., Horaud T.;
RT   "Nucleotide sequence of the chloramphenicol resistance determinant of
RT   the streptococcal plasmid pIP501";
RL   Plasmid 28:272-276(1992).
XX
RN   [2]
RP   1-901
RA   Trieu-Cuot P.;
RT   ;
RL   Submitted (07-APR-1992) to the EMBL/GenBank/DDBJ databases.
RL   P. Trieu-Cuot, Institut Pasteur, Lab des Staphylocoques et, des
RL   Streptocoques, 25 Rue du Dr Roux, F 75724 Paris Cedex, FRANCE
XX
DR   SWISS-PROT; P36884; LPCA_STAAU.
DR   SWISS-PROT; Q03058; CAT_STRAG.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..901
FT                   /organism="Streptococcus agalactiae"
FT                   /plasmid="Plasmid"
FT                   /strain="B96"
FT   -35_signal      91..96
FT   -10_signal      113..118
FT   CDS             144..173
FT                   /db_xref="PID:g581554"
FT                   /db_xref="SWISS-PROT:P36884"
FT                   /product="leader peptide"
FT                   /translation="MKKSEDYSS"
FT   CDS             208..855
FT                   /db_xref="PID:g49072"
FT                   /db_xref="SWISS-PROT:Q03058"
FT                   /product="chloramphenicol acetyltransferase"
FT                   /gene="cat"
FT                   /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKKK
FT                   GYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWTES
FT                   DKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNSSFL
FT                   LPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
FT   attenuator      163..200
XX
SQ   Sequence 901 BP; 353 A; 89 C; 135 G; 324 T; 0 other;
     TCGACTTTAA AAGCGAACGA AAAACAATTG CAAAAGCAGA TTGATTTTTG GCAACGTGAA        60
     TTTAGGTTTT GGAAGTAAAA TAAGTTTTAT TTGATAAAAA TTGCTAATTC AGTATAATTA       120
     ATATTTACGA GGTGACATAA CGTATGAAAA AATCAGAGGA TTATTCCTCC TAAATATAAA       180
     AATTTAAAAT TTAGGAGGAA GTTATATATG ACTTTTAATA TTATTGAATT AGAAAATTGG       240
     GATAGAAAAG AATATTTTGA ACACTATTTT AATCAGCAAA CTACTTATAG CATTACTAAA       300
     GAAATTGATA TTACTTTGTT TAAAGATATG ATAAAAAAGA AAGGATATGA AATTTATCCC       360
     TCTTTAATTT ATGCAATTAT GGAAGTTGTA AATAAAAATA AAGTGTTTAG AACAGGAATT       420
     AATAGTGAGA ATAAATTAGG TTATTGGGAT AAGTTAAATC CTTTGTATAC AGTTTTTAAT       480
     AAGCAAACTG AAAAATTTAC TAACATTTGG ACTGAATCTG ATAAAAACTT CATTTCTTTT       540
     TATAATAATT ATAAAAATGA CTTGCTTGAA TATAAAGATA AAGAAGAAAT GTTTCCTAAA       600
     AAACCGATAC CTGAAAACAC CATACCGATT TCAATGATTC CTTGGATTGA TTTTAGTTCA       660
     TTTAATTTAA ATATTGGTAA CAATAGCAGC TTTTTATTGC CTATTATTAC GATAGGTAAA       720
     TTTTATAGTG AGAATAATAA AATTTATATA CCAGTTGCTC TGCAACTTCA TCATTCTGTA       780
     TGTGATGGTT ACCATGCTTC ACTATTTATG AATGAATTTC AAGATATAAT TCATAGGGTA       840
     GATGATTGGA TTTAGTTTTT AGATTTTGAA AGTGAATTTA ATTTTATACA CGTAAGTGAT       900
     C                                                                       901
//