- Access to the animal facility is restricted to persons authorized by the responsible person and advised of the potential risks. An access control system is put in place.
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- The room access door to the animal facility is labelled with the following information:
- Biohazard symbol,
- Containment level,
- Coordinates of the responsible person for the area,
- Nature of the biological risk,
- The list of persons authorized to enter the area,
- Requirements for entering the contained area.
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- Dedicated equipment is assigned to the animal facility.
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- Protective clothing is worn. Protective clothing is dedicated to the contained area and is not worn outside. It is decontaminated in the contained area prior to laundering or elimination.
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- Gloves are available for the personnel and worn when necessary.
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- The creation of splashes and the formation of aerosols are minimized. Their spreading is controlled by the use of appropriate equipment and practices.
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- Use of a horizontal airflow cabinet is prohibited for the manipulation of pathogens and/or genetically modified (micro)-organisms and contaminated animals.
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- Mechanical pipetting devices are used. Mouth pipetting is prohibited.
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- Eating, drinking, smoking, handling contact lenses, applying cosmetics, and storing food for human consumption are not permitted in the room.
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- Registers record all the operations that have been performed (animals entries and exits, inoculations to animals of pathogens and/or genetically modified (micro)-organisms, etc.).
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- Control measures and control equipment as well as protective equipment are adequately and regularly tested.
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- The workers wash their hands when they leave the contained area, before beginning another activity and each time it is proved necessary.
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- Work surfaces are decontaminated with an appropriate disinfectant after work is finished and after any accidental spill of biological material.
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- Directions for use of disinfectants are available for the personnel. Depending on the purpose, instructions precise the kind of disinfectant to use, its concentration, and contact time.
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- Siphons are protected with disinfectant.
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- Instruction of the personnel on biosafety aspects is organized as well as a follow up and regular updates. The personnel are specifically trained to work in an area with containment level 3.
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- A biosafety manual is prepared and adopted. Personnel are advised of special risks they are exposed to and are required to read instructions on work practice. Behaviour in case of accident is clearly posted in the room.
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- The biosafety symbol is posted on incubators, refrigerators, freezers, and liquid nitrogenous cryopreservators containing biological material with a class of risk 2 or higher.
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- An efficient control program of potential vectors such as rodents and insects is in effect.
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- Animals that take part to the experiment are isolated in a separate room.
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- Management of wastes and/or residual biological material satisfies the following conditions:
- Contaminated wastes and/or residual biological material (carcasses, excrements, contaminated bedding, etc.) and contaminated disposal are inactivated by an appropriate and validated method before disposal, (e.g. by autoclaving or incineration). Incineration is performed in an agreed installation. Bags and containers used for infectious waste collect are resistant, sealable, labelled with the biosafety symbol and closed before leaving contained area.
- Before washing, reuse and/or destruction, contaminated material (glassware, cages, etc.) is inactivated by an appropriate and validated means.
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- Effluents from sinks and showers are preferably inactivated by an appropriate and validated means before final evacuation.
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