- Access to the laboratory is restricted to persons authorized by the responsible person and advised of the potential risks. An access control system is put in place.
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- The room access door is labelled with the following information:
- Biohazard symbol and Containment level,
- Coordinates (name and phone number) of the responsible person for the area,
- Nature of the biological risk,
- The list of persons authorized to enter the area,
- Requirements for entering the contained area.
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- Dedicated equipment is assigned to the laboratory.
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- Protective laboratory clothing is worn. Protective clothing is dedicated to the contained area and is not worn outside. It is decontaminated preferably in the contained area prior to laundering or elimination.
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- Gloves are available for personnel and worn when necessary.
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- Outside manipulations, viable pathogens and/or genetically modified (micro)-organisms are contained within closed systems (tubes, flasks, etc.).
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- The creation of splashes and the formation of aerosols are minimized. Their spreading is controlled by the use of appropriate equipment and practices.
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- All manipulations likely to produce infectious aerosols or involving potential risks are conducted within a biological safety cabinet.
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- Use of a horizontal laminar flow cabinet is prohibited for the manipulation of pathogens and/or genetically modified (micro)-organisms.
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- Mechanical pipetting devices are used. Mouth pipetting is prohibited.
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- Eating, drinking, smoking, handling contact lenses, applying cosmetics, and storing food for human consumption are not permitted in the laboratory.
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- All the manipulated and stored pathogens and/or genetically modified (micro)-organisms are recorded in a register.
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- Control measures and control equipment as well as protective equipment are adequately and regularly tested.
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- Workers wash their hands when they leave the contained area, before beginning another activity and each time it is proved necessary.
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- Work surfaces are decontaminated with an appropriate disinfectant after work is finished and after any spill of biological material.
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- Directions for use of disinfectants are available for the personnel. Depending on the purpose, instructions prescribe the kind of disinfectant to use, its concentration and contact time.
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- Instruction of personnel on biosafety aspects is conducted as well as a follow up and regular updates. The personnel are specifically trained to work in an area with containment level 3.
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- A biosafety manual is prepared and adopted. Personnel are advised of special risks they are exposed to and are required to read instructions on work practice. Behaviour in case of an accident is clearly posted in the laboratory.
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- The biosafety symbol is posted on incubators, refrigerators, freezers, and liquid nitrogen cryopreservators containing biological material with a BSL-2 or higher.
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- An efficient control program for rodents and insects is in effect.
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- Animals are not allowed in the laboratory except as part of experiments.
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- Management of wastes and/or residual biological material satisfies the following conditions:
- Contaminated wastes and/or residual biological material and contaminated disposal are inactivated by an appropriate and validated method before disposal, (e.g. by autoclaving or incineration). Incineration is performed in an accredited installation. Bags and containers used for infectious waste collection are resistant, sealable, labelled with the biosafety symbol and closed before leaving the contained area.
- Before washing, reuse and/or destruction, contaminated material (glassware, slides, etc.) is inactivated by appropriate and validated means.
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- Effluents from sinks and showers are preferably inactivated by appropriate and validated means before final disposal.
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