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BELGIAN BIOSAFETY SERVER
Biosafety Recommendations for the Contained Use of Mycobacterium tuberculosis Complex Isolates in Industrialised Countries
Author: P. Herman , M. Fauville-Dufaux , D. Breyer , B. Van Vaerenbergh , K. Pauwels , C. D. Do thi , M. Sneyers , M. Wanlin , R. Snacken , W. Moens (Last revised:
September 12, 2005 )
Table 6: Biosafety level 3 - Laboratory Facilities: Safety Equipment.
- The laboratory has at least one biological safety cabinet if open manipulations are performed. It is installed to avoid disturbing airflow equilibrium inside the work area. It is located away from doors, windows, room supply and exhaust air louvers, and heavily travelled laboratory areas. It is controlled and certified when placed, after each moving, and at least once a year.
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- An autoclave, preferably a double-door one, is located in contained area.
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- Biological material is centrifuged in centrifuges located in the contained area. It is placed in leak-proof tubes in rotors or cups with a hermetic closing system ("safety cups") to contain aerosols in case of breaking or cracks in the tubes.
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- Vacuum lines used for work are provided with HEPA filters.
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