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1. Flow Cytometry - Sample handling
(Author: P. Herman) (Last revised: November 26, 2014 )


Knowledge of the origin of the sample and awareness of the potential presence of pathogens (genetically modified or not) are keys for lab scientists' protection.

• All unfixed materials (blood samples, various body fluids, cultured cells and environmental samples) should be a priori considered as biohazardous (the use of a fixative is not always sufficient to eliminate all type of biohazards);

• The use of Class II biological safety cabinet for sample preparation is highly recommended;

• Flow cytometer operators should be experienced and well trained;

• in relation with the Biosafety status of the sample: appropriate containment level, protective equipment, work practices and waste management are adopted after a thorough risk assessment (according to the European Directive 2009/41/EC repealing Directive 90/219/EEC, European Directive 2000/54/EC).

See also laboratory techniques that produce aerosols and/or droplets
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