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1. Flow
Cytometry - Sample
handling
(Author: P. Herman) (Last revised:
July 10, 2006
)
Knowledge of the origin of the sample and awareness of the potential presence of pathogens and/or GMOs are keys for lab scientists' protection.
• All unfixed materials (blood samples, various
body fluids, cultured cells and environmental samples) should be a priori considered as biohazardous (The use of a fixative is not always sufficient to eliminate all type of biohazards). • The use of CLASS II biological safety cabinet
for sample preparation is highly recommended. • Flow cytometer operators should be experienced
and well trained. • Appropriate containment level must be applied
(according to the Directive 98/81/EC) in relation with the Biosafety status of the sample.
 See laboratory techniques that produce aerosols in chapter 2 : Flow generated aerosols and/or droplets
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