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3. Flow
Cytometry -
Waste Management and Equipment Maintenance
(Author: P. Herman) (Last revised:
July 10, 2006
)
The procedures of waste management and equipment maintenance must be adapted to the biosafety status of the handled biological material.
Waste management : Usually, the effluent are collected from the instrument in a flask containing fresh concentrated bleach to achieve a 10% final concentration. The Inactivation method of the effluent must be appropriate and validated. The contents of the flask is disposed according to institutional and local regulations for waste disposal.
Maintenance of fluid lines and instrument parts : Fluid lines desinfection requires regular instrument runs with a 1:10 fresh dilution of a 5.25% sodium hypochlorite solution for at least 10 minutes, followed by distilled water.
Flow cytometer's parts : follow the manufacturer's recommendations for appropiate desinfection.
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