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Animal
cell cultures
(Last revised:
February 28, 2006
)
Conditions to be fulfilled in order to consider cells free
of adventitious contaminating pathogens:
- the use of well-characterized cell lines or controlled cell sources for primary cells such as specified-pathogen- free (SPF) animals. If no well-characterized cell lines or SPF are available, tests for detection of likely contaminating agens should be negative;
- the use of media sources free from contamination;
- the use of appropriate containment measures to reduce contaminations during sample take or subsequent manipulation of cells (refeeding, washing steps).
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