Specifications	Containment levels
	1	2	3	4

1	Laboratory suite: isolation (1)	Not required	Not required	Required	Required
2	Laboratory: sealable for fumigation	Not required	Not required	Required	Required

Equipment

3	Surfaces resistant to water, acids, alkalis, solvents, disinfectants, decontamination agents and easy to clean	Required (bench)	Required (bench)	Required (bench, floor)	Required (bench, floor, ceiling, walls)
4	Entry to lab via airlock (2)	Not required	Not required	Optional	Required
5	Negative pressure relative to the pressure of the immediate environment	Not required	Not required	Required except for (3)	Required
6	Extract and input air from the laboratory should be HEPA-filtered	Not required	Not required	Required (HEPA) (4) - extract air except for (3)	Required (HEPA) (5) - input and extract air
7	Microbiological safety post	Not required	Optional	Required	Required
8	Autoclave	On site	In the building	En suite (6)	In lab = double-ended

System of work

9	Restricted access	Not required	Required	Required	Required
10	Biohazard sign on the door	Not required	Required	Required	Required
11	Specific measures to control aerosol dissemination	Not required	Required minimise	Required prevent	Required prevent
13	Shower	Not required	Not required	Optional	Required
14	Protective clothing	Suitable protective clothing	Suitable protective clothing and (optional) footwear	Suitable protective clothing	Complete change of clothing and footwear before entry and exit
15	Gloves	Not required	Optional	Required	Required
18	Efficient vector control (e.g. for rodents and insects)	Optional	Required	Required	Required

Waste

19	Inactivation of GMMs in effluent from hand-washing sinks or drains and showers and similar effluents	Not required	Not required	Optional	Required
20	Inactivation of GMMs in contaminated material and waste	Optional	Required	Required	Required

Other measures

21	Laboratory to contain its own equipment	Not required	Not required	Optional	Required
23	An observation window or alternative is to be present so that occupants can be seen	Optional	Optional	Optional	Required

(1) Isolation = the laboratory is separated from other areas in the same building or is in a separated building
(2) Airlock = entry must be through an airlock which is a chamber isolated from the laboratory. The clean side of the airlock must be separated from the restricted side by changing or showering facilities and preferably by interlocking doors.
(3) Activities where transmission does not occur via airborne route.
(4) HEPA = High efficiency particulate air.
(5) Where viruses which are not retained by HEPA filters are used, extra requirement will be necessary for extract air.
(6) With validated procedure, allowing the safe transfer of material into an autoclave outside the lab, and providing an equivalent level of protection.