BELGIAN BIOSAFETY SERVER : Directive 90/220/EEC
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ANNEX I A

TECHNIQUES REFERRED TO IN ARTICLE 2 (2)

PART 1

Techniques of genetic modification referred to on Article 2 (2) (i) are inter alia:

(1) recombinant DNA techniques using vector systems as previously covered by Council Recommendation 82/472/EEC(1);

(2) techniques involving the direct introduction into an organism of heritable material prepared outside the organism including micro-injection, macro-injection and micro-encapsulation;

(3) cell fusion (including protoplast fusion) or hybridization techniques where live cells with new combinations of heritable genetic material are formed through the fusion of two or more cells by means of methods that do not occur naturally.

PART 2

Techniques referred to in Article 2 (2) (ii) which are not considered to result in genetic modification, on condition that they do not involve the use of recombinant DNA molecules or GMOs, are:

(1) in vitro fertilization,

(2) conjugation, transduction, transformation or any other natural process,

(3) polyploidy induction.

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(1) OJ No L 213, 21.7.1982, p.15

BELGIAN BIOSAFETY SERVER : Directive 90/220/EEC
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