The measures contained in this Annex shall be applied according to the nature of the activities, the assessment of risk to workers, and the nature of the biological agent concerned.

A. Containment measures	B. Containment levels
	2	3	4
1. The workplace is to be separated from any other activities in the same building	No	Recommended	Yes
2. Input air and extract air to the workplace are to be filtered using (HEPA) or likewise	No	Yes, on extract air	Yes, on input and extract air
3. Access is to be restricted to nominated workers only	Recommended	Yes	Yes, via airlock
4. The workplace is to be sealable to permit disinfection	No	Recommended	Yes
5. Specified disinfection procedures	Yes	Yes	Yes
6. The workplace is to be maintained at an air pressure negative to atmosphere	No	Recommended	Yes
7. Efficient vector control e.g. rodents and insects	Recommended	Yes	Yes
8. Surfaces impervious to water and easy to clean	Yes, for bench	Yes, for bench and floor	Yes, for bench, walls, floor and ceiling
9. Surfaces resistent to acids, alkalis, solvents, disinfectants	Recommended	Yes	Yes
10. Safe storage of a biological agent	Yes	Yes	Yes, secure storage
11. An observation window, or, alternative, is to be present, so that occupants can be seen	Recommended	Recommended	Yes
12. A laboratory is to contain own equipment	No	Recommended	Yes
13. Infected material including any animal is to be handled in a safety cabinet or isolator or other suitable containment	Where appropriate	Yes where infection is by airborne route	Yes
14. Incinerator for disposal of animal carcases	Recommended	Yes (available)	Yes, on site